Shenyang Agricultural University
Lilium Quality Control and Germplasm Innovation Research Team
The research team of lily quality control and germplasm innovation of Shenyang Agricultural University is belong to the key laboratory of Ministry of Education (provincial total construction) for the facilities horticulture, also a member of the National Local Joint Engineering Research Center for the Design and Application Technology of Northern Horticultural facilities. The research team was engaged in lily research for more than 20 years and has undertaken more than 30 research projects at the provincial and ministerial level, of which 8 are funded by the National Natural Science Foundation and 1 by the State key R & D projects. Many awards were obtained including 2 second prize for science and technology progress award of Liaoning province, 2 first prize and 3 second prize for natural science academic achievement of Liaoning province. Fifteen National Invention Patents and 9 Utility Model Patents have been applied for and obtained. More than 200 academic papers, 6 editor-in-chief books and more than 20 collaboratively writed monographs were published. The team now has 2 professors, 1 associate professor, 3 lecturers, 11 doctoral students and 19 master students. Professor Sun Hongmei, team leader, doctoral supervisor, is mainly engaged in lily bulb quality regulation and the mechanism of bulb development and dormancy. Professor Sun Xiaomei, doctoral supervisor, mainly focuses on lily breeding and hybrid breeding. Associate professor Chen Lijing, doctoral supervisor, mainly carries on the research of cold resistance mechanism and germplasm innovation of lily. Lecturer Wang Jinxia dose the research on lily efficient cultivation under facility conditions. Lecturer Wang Chunxia is engaged in the research of lily resource collection and evaluation. Lecturer Wang Zhiping specialize in genome editing technology and gene enhanced breeding.
The mechanism of the development and dormancy of lily bulbs: Spatial analysis of the metabolism of lily scales was carried out. The space-time pattern and the change rule of the starch, the sucrose metabolism during the development and low-temperature storage of the lily bulb were determined. From the time-space expression of the key enzyme genes, the mechanism of the degradation of the sucrose in the process of the starch metabolism of the lily bulbs was preliminarily clarified.
The cultivation technique of superior quality lily bulbs: Different kinds of high efficiency regeneration and in vitro rapid propagation technology systems were established, such as regeneration through adventitious bud induction, direct regeneration in the form of bulblets, indirect regeneration through callus, regeneration through somatic embryogenesis. The low-temperature storage model, the quality evaluation and testing technology systems of lily bulbs were successfully built.
The mechanism and application of somatic embryogenesis in lily: The expression profiles of miRNAs and their target genes in different stages of somatic embryogenesis were constructed, and the miRNA regulatory network related to somatic embryogenesis in lily was preliminarily constructed. An efficient genetic transformation system based on somatic embryogenesis was established and gene editing technique was successfully applied to lily. Many new polyploid germplasm of wild lilies were created.
The selecting and breeding of new variety of lily: New varieties of lily with various plant types and a new variety of Lilium longiflorum which can bloom twice a year were bred by carrying out multi-generation hybridization. A new derived variety of Lilium regale Wilson with fragrance and gradient color was cultivated, also a batch of new Asian lily varieties suitable for cutting flowers, ground flora and edible were created.